Diploneis calcicolafrequens has narrower valves. Diploneis hinziae has more elliptical valves with bluntly rounded apices and biseriate striae in the marginal parts. Diploneis parahinziae has linear-elliptical valves with broadly rounded ends and higher striae- and areolae density (striae 12.5–14 and areolae 15–20 in 10 μm). Diploneis elliptica has a larger size range and expanded central area. Small valves of both species, however, are quite similar and differ only in stria density. Valves at the small end of the size range of these species should be carefully examined.