Stauroneis bryocola has lanceolate valves with subtly protracted and broadly rounded apices. Stauroneis gracilior, S. kootenai, and S. pikuni have lanceolate valves with capitate apices and a lower stria and areola density (< 30 in 10 µm). Stauroneis schroederi has lanceolate valves with gradually attenuated apices and striae and areolae <30 in 10 µm. Stauroneis subgracilior has smaller, lanceolate valves.