Stephanodiscus niagarae is smaller and has a higher areola density (11-20 in 10 µm) than S. reimeri, which is larger and has 8-12 areolae in 10 µm.
Stephanodiscus alpinus has spines at the end of nearly every costa and the interfascicles are 8-11 in 10 µm.
Stephanodiscus neoastraea differs by having scattered areolae in the center, lacks central fultoportulae and the interfascicles are 7-9 in 10 µm.
Stephanodiscus niagarae differs from S. yellowstonensis by having greater numbers of spines at similar valve diameter and more regular spine placement on every second or third costae versus spines on every second to fifth costae in S. yellowstonensis.