Aulacoseira valida is sometimes confused with large specimens of A. ambigua. Aulacoseira ambigua has spines at the end of each pervalvar costa. Linking spines are short, triangular or bifurcated.
Aulacoseira italica has mantle areolae that are almost straight or slightly curved. The rows of areolae are either parallel to the pervalvar axis or inclined to the left .