Description: Molecular phylogenetic data are crucial for obtaining a deeper understanding of the diversity, evolutionary history and biogeography of diatoms. In this talk, Pinnularia borealis is used as a case study to demonstrate the power of molecular phylogenetics for resolving a cryptic species complex. The talk will outline a typical workflow to obtain and apply molecular data: from sampling live diatoms, to culture work, marker choice, and molecular species delimitation. Several techniques to study patterns in diatom diversity will be highlighted, using P. borealis as an exemplar, including time-calibration of phylogenies and analyses on historical biogeography. A clear link with morphological data will be given throughout the talk.

Target Audience: The talk is aimed at anyone interested in diatom phylogenetics, cryptic diversity, species delimitation, and biogeography. It will be of particular interest to researchers who wish to apply molecular phylogenetic data in their research, or who simply wish to learn more about these techniques.